LAB REPORT
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LAB REPORT
Discussion
Lab Report
Discussion
While we are not excessively simplistic in our estimates due to uncertainty in the development of our criteria and some somewhat muddled emails that made it much more complicated, we still received responses that were reasonable. We were able to successfully calculate the purity of our deuterated caffeine sample, as well as predict the concentration of caffeine in our unknown caffeine sample. Although there have been some experimental errors that have blocked a particular analysis technique for us, the multidimensional nature of GC to MS has allowed us to do something else (comparing all mass spectrograph signal ratios to a single calibration standard signal ratio). Due to our lack of confidence in our estimates, our data may not be especially reliable or precise. However, we have still been able to collect information on the use of GC to MS as an analysis tool. We were capable of calculating the properties of our three samples, despite the occurrence of errors in which we did not hold the concentration of one of our caffeine samples constant while serially diluting it, causing us to have three samples of constant caffeine; d3 – caffeine ration instead of three samples with a ratio that varies, which resulted in us in shifting and using alternative means of calculation in determining the properties of our sample (Panek-Shirley et al. 2016). However, perhaps the most important of our forecasts is that our predicted content is based on experimental values, whereby the majority of forms of co-operation varies from 100 to 200 ppm caffeine.
Conclusion
The caffeine content in non-alcoholic beverages and energy drinks is an important analytical process that protects the well-being of those unaware of the adverse impacts of caffeine (Smith & Dill 1988). A fast, precise, accurate and low-cost method for the determination of caffeine in coffee beans has been developed in the current work without the use of an internal standard. The method was considered “fit for purpose” in accordance with the requirements of ISO 17025 and the European Regulation on aspects related to the accurate development of analytical methods. The direct injection method developed for caffeine analysis of sugar-free beverages via GC-MS can be considered relatively fast, easy, and reliable. No costly solvents and reagents are required, and therefore it can be recommended for rapid, accurate and sensitive caffeine quantification in drinks. Five-hour energy containing the highest content of caffeine was investigated among drinks.
The method used to extract caffeine from tea and coffee samples was effective. The process can potentially be used for the extraction and determination of different compounds in a variety of natural products (Solomon et al. 1997). Caffeine is widely consumed because of its ability to improve alertness and thinking efficiency, but it is important for consumers to know the caffeine content of commonly consumed drinks as high caffeine intake may have health implications.
References
Panek-Shirley, L. et al., 2016. The independent and combined effects of caffeine and exercise on eating behaviour, pp.ProQuest Dissertations and Theses.
Smith, M. & Dill, Charles A., 1988. Psychological effects of acute and chronic caffeine intake in humans, pp.ProQuest Dissertations and Theses.
Solomon, R. & Schare, Mitchell L., 1997. The effects of trait anxiety, daily consumption level and a dose of caffeine on female office workers, pp.ProQuest Dissertations and Theses.