Introduction and Review of Literature
Number of head and neck cancer cases and deaths reported Worldwide. Source: GLOBOCAN 2018 (Bray F et al., 2018)
Number of reported cancers in Assam according to Population-based cancer registry (PBCR) data of 2012-14.
Oral Bacteria as a diagnostic and prognostic biomarker of HNSCC
Molecular mechanisms of oral bacteria associated with HNSCC
Alterations of predominant oral bacteria identified in HNSCC
Association between oral bacteria and HNSCC in clinical studies (Source: Al-Hebshi NN et al. 2019)
Characteristic features of next-generation sequencing technologies
Materials and Methods
List of chemicals and kits used in the study
List of Primers used in the study
Sample size determination methods
The selected sample size for the study
The selected sample size for the discovery phase and functional validation phase study
The following reaction was setup for amplifying the genomic DNA
Bioinformatics and statistics software or tools used for the present study
Results and Discussions
Section-I: Demographic characteristics of head and neck cancer cases and controls
Descriptive data of the demographic characteristics study population. Information about age, gender, oral habits, tumor stage and grade is provided
Summarized demographic characteristics of the HNSCC cases and controls (N=100)
Denoting age group study in HNSCC patients and healthy controls
Mean and median of age group study in HNSCC patients and healthy controls
Denoting gender of the HNSCC patients and healthy controls
Total number of males and females in the group of HNSCC cases and healthy controls
History of oral habits of HNSCC patients and healthy controls
Denoting the stage of HNSCC patients
Denoting the grade of HNSCC patients
Results and Discussions
Section-II: Comparison of salivary bacteriome profiles in between controls and oropharyngeal & hypopharyngeal cancer cases
A summary of the demographic and clinical characteristics of OPSCC patients
A summary of the demographic and clinical characteristics of HPSCC patients
A summary of the demographic characteristics of the control subjects
The ladder and sample details for each lane in both Figure-1 and Figure-2
Read count statistics for oropharyngeal cancer (OPC) samples of V3-V4 region (Panda et al., 2019)
Read count statistics for hypopharyngeal cancer (HPC) samples of V3-V4 region (Panda et al., 2019)
Read count statistics for healthy control (C) samples of V3-V4 region (Panda et al., 2019)
Functional diversity index of oropharyngeal cancers samples at a depth of 80,000 sequences/sample calculated using QIIME for V3-V4 samples (Panda et al., 2019)
Functional diversity index of hypopharyngeal cancers samples at depth of 80,000 sequences/sample calculated using QIIME for V3-V4 samples (Panda et al., 2019)
Functional Diversity index of controls at depth of 80,000 sequences/sample calculated using QIIME for V3-V4 samples (Panda et al., 2019)
Statistically significant elevated bacterial species in oropharyngeal cancer patients (Panda et al., 2019).
Statistically significant reduced bacterial species in OPC patients (Panda et al., 2019)
Statistically significant elevated bacterial species in HPC patients (Panda et al., 2019)
Statistically significant reduced bacterial species in HPC patients (Panda et al., 2019)
Abundant and Rare Species in Control and OP Cancer Patients (Panda et al., 2019).
Results and Discussions
Section-III: Comparison of salivary bacteriome profiles in between controls and oral squamous cell carcinoma (OSCC) cases
Results and Discussions
Section-IV: Comparison of salivary bacteriome profiles in between controls & cases
having oral pre-malignant lesion (OPML) and comparison of OPML cases with OSCC
cases