Doxorubicin
Doxorubicin is a chemotherapeutic agent with genotoxic effect on normal cells at therapeutic dose itself. D-Pinitol is an abundantly available carbohydrate in Soyabean plants and was proved for its antiinflammatory, antioxidant and anticancer activities. The study was aimed to explore whether D-Pinitol protects normal cells and tissues against Doxorubicin-induced genotoxicity in Vero cell line and Swiss albino mice by invitro comet assays. Vero cell lines were seeded in a six-well plate (10,000 cells/well): a control group, doxorubicin alone treated group, three D-Pinitol alone treated groups and three D-Pinitol and Doxorubicin simultaneously treated groups. Swiss albino mice were treated: a control group, doxorubicin alone treated group, four D-Pinitol alone treated groups, four D-Pinitol and Doxorubicin simultaneously treated groups. The results showed that doxorubicin-induced a noticeable genotoxic effect in Vero cell lines. D-Pinitol reduced the effects of doxorubicin significantly in both types of experimental conditions, through reduced the level of DNA damage measured via invitro comet assay. Our results suggest a protective effect of D-Pinitol against Doxorubicin-induced genotoxicity. The most protective effect was observed with higher doses of D-Pinitol before doxorubicin treatment.