experimental research that involves the manipulation of variables.

experimental research that involves the manipulation of variables.

The paper presents experimental research that involves the manipulation of variables. The variables are measured, calculated, and compared to establish their relationships or effect. The research is also experimental because it is performed in a controlled environment which is a lab. In this experimental research, the authors present a practical PCR-based H-typing system and H-genotyping PCR for E. coli. Additionally, they involve various materials and methods in the experiment. For example, the methods and experiments include source sequences, sequence comparison, bacterial strains, simplex PCR, as well as multiplex PCR. From the experiment, the results indicate that, however, serotyping is a standard technique for subtyping the isolates of pathogenic E. coli, the clumping together of the reactions with specific antisera are cumbersome and time-consuming.

A follow up involves the quasi-experimental. In this experiment, there can be a process needed in the control experiment that is missing hence it is unable to be completed. Particularly, it has a close relationship with the randomization process. There is no manipulation of the independent variable in this follow up experiment. The major objective of this experiment is to identify the variables that are to remain constant while observing the effect of variation on other variables of interests. Nevertheless, the experiments undertaken by the authors have various strengths, for instance, it is easier to replicate the laboratory experiment. It is as a result of a standardized procedure that is used by the authors. Moreover, they allow for precise control of extraneous as well as independent variables. This allows the establishment of a cause and effect relationship. in this case, the experiment presents a basic set of PCR primers for the process of identification of all known E. coli H types and also confirms that the complete, as well as a practical platform for molecular H typing and E. coli H-genotyping, can accurately identify the H types.